A question about nanopore sequencing analysis

Muhammad Danie Al Malik

@daniealmalik
| danie

I am highly committed to developing my career in the field of marine ecology, taking part in solving the challenges on biodiversity preservation in the Indonesian region

Groups

eDNA & Genomics

Hello,

I am from Indonesia, and currently, I am researching the eDNA technique using nanopore sequencing to detect several marine taxonomic. Do you know how to reduce unclassified barcodes from the guppy process (basecalling)?

Best regards,

Chris Yesson

@CYesson

Zoological Society London (ZSL)

I am a benthic ecologist using low-cost tech to explore seabed habitats in temperate and cold water.

25 November 2024 11:18am

Not an expert here, but when I used this a few years back we initially used the guppy process to do real-time base calling as the nanopore was sequencing, but found that we were better off waiting for the sequencing to finish and then reprocessing all the output using the slow and careful option of the guppy base-calling process (which was way to slow to do effective real-time calling).

Nabil Amor

@nabil.amor

My research focuses on using Next-Generation Sequencing (NGS) to study endangered species, including carnivores, chiropterans (bats), and lizards, as well as their microbiomes.

6 January 2025 9:18pm

Hi Muhammed,

If you want any help with ONT please contact me